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web-based multiplex pcr primer design software ultiplex  (PrimerDesign Inc)

 
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    PrimerDesign Inc web-based multiplex pcr primer design software ultiplex
    Diagram of the <t>Ultiplex</t> workflow
    Web Based Multiplex Pcr Primer Design Software Ultiplex, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/web-based multiplex pcr primer design software ultiplex/product/PrimerDesign Inc
    Average 90 stars, based on 1 article reviews
    web-based multiplex pcr primer design software ultiplex - by Bioz Stars, 2026-06
    90/100 stars

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    1) Product Images from "The web-based multiplex PCR primer design software Ultiplex and the associated experimental workflow: up to 100- plex multiplicity"

    Article Title: The web-based multiplex PCR primer design software Ultiplex and the associated experimental workflow: up to 100- plex multiplicity

    Journal: BMC Genomics

    doi: 10.1186/s12864-021-08149-1

    Diagram of the Ultiplex workflow
    Figure Legend Snippet: Diagram of the Ultiplex workflow

    Techniques Used:

    Filtration functions of Ultiplex. Primers need to be filtered with different functions to eliminate malfunctional pairs. A Getprimers.harpin_filter function. If there is a hairpin structure in the combined sequence of the primer and tag, the primer pair will be eliminated. B Getprimers.dimer_filter function. If there is a dimer structure between any two combined primer and tag sequences, the primer pair will be eliminated. C Getprimers.area_filter & Getprimers.site_filter functions. If any skipped sites or areas are located at the 3′ end of the primer, the primer pair will be eliminated. D Getprimers.single_blast_filter function. The potential binding sites of a single primer are evaluated, and if the amplicon length is below the cut-off value, the primer pair will be eliminated
    Figure Legend Snippet: Filtration functions of Ultiplex. Primers need to be filtered with different functions to eliminate malfunctional pairs. A Getprimers.harpin_filter function. If there is a hairpin structure in the combined sequence of the primer and tag, the primer pair will be eliminated. B Getprimers.dimer_filter function. If there is a dimer structure between any two combined primer and tag sequences, the primer pair will be eliminated. C Getprimers.area_filter & Getprimers.site_filter functions. If any skipped sites or areas are located at the 3′ end of the primer, the primer pair will be eliminated. D Getprimers.single_blast_filter function. The potential binding sites of a single primer are evaluated, and if the amplicon length is below the cut-off value, the primer pair will be eliminated

    Techniques Used: Filtration, Sequencing, Binding Assay, Amplification



    Similar Products

    web-based multiplex pcr primer design software ultiplex  (PrimerDesign Inc)
    90
    PrimerDesign Inc web-based multiplex pcr primer design software ultiplex
    Diagram of the <t>Ultiplex</t> workflow
    Web Based Multiplex Pcr Primer Design Software Ultiplex, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/web-based multiplex pcr primer design software ultiplex/product/PrimerDesign Inc
    Average 90 stars, based on 1 article reviews
    web-based multiplex pcr primer design software ultiplex - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier

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    Diagram of the Ultiplex workflow

    Journal: BMC Genomics

    Article Title: The web-based multiplex PCR primer design software Ultiplex and the associated experimental workflow: up to 100- plex multiplicity

    doi: 10.1186/s12864-021-08149-1

    Figure Lengend Snippet: Diagram of the Ultiplex workflow

    Article Snippet: Here, we developed the web-based multiplex PCR primer design software “Ultiplex”.

    Techniques:

    Filtration functions of Ultiplex. Primers need to be filtered with different functions to eliminate malfunctional pairs. A Getprimers.harpin_filter function. If there is a hairpin structure in the combined sequence of the primer and tag, the primer pair will be eliminated. B Getprimers.dimer_filter function. If there is a dimer structure between any two combined primer and tag sequences, the primer pair will be eliminated. C Getprimers.area_filter & Getprimers.site_filter functions. If any skipped sites or areas are located at the 3′ end of the primer, the primer pair will be eliminated. D Getprimers.single_blast_filter function. The potential binding sites of a single primer are evaluated, and if the amplicon length is below the cut-off value, the primer pair will be eliminated

    Journal: BMC Genomics

    Article Title: The web-based multiplex PCR primer design software Ultiplex and the associated experimental workflow: up to 100- plex multiplicity

    doi: 10.1186/s12864-021-08149-1

    Figure Lengend Snippet: Filtration functions of Ultiplex. Primers need to be filtered with different functions to eliminate malfunctional pairs. A Getprimers.harpin_filter function. If there is a hairpin structure in the combined sequence of the primer and tag, the primer pair will be eliminated. B Getprimers.dimer_filter function. If there is a dimer structure between any two combined primer and tag sequences, the primer pair will be eliminated. C Getprimers.area_filter & Getprimers.site_filter functions. If any skipped sites or areas are located at the 3′ end of the primer, the primer pair will be eliminated. D Getprimers.single_blast_filter function. The potential binding sites of a single primer are evaluated, and if the amplicon length is below the cut-off value, the primer pair will be eliminated

    Article Snippet: Here, we developed the web-based multiplex PCR primer design software “Ultiplex”.

    Techniques: Filtration, Sequencing, Binding Assay, Amplification